|
| Status |
Public on Feb 04, 2020 |
| Title |
ky-E2 |
| Sample type |
RNA |
| |
|
| Source name |
Kyse150-neo-EN2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Kyse150
genotype: overexpression of the EN2
cell type: esophageal squamous cell carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol (Invitrogen, Carlsbad, CA, USA), and a total of 500 ng RNA was used for cDNA synthesis
|
| Label |
biotin
|
| Label protocol |
sample was labeled with biotin by terminal deoxynucleotidyl transferase (TdT)
|
| |
|
| Hybridization protocol |
sample was hybridized to the Affymetrix Clariom S Assay,human (Cat No 902926) for 16-18 h at 45°C. Following hybridization, the microarrays were washed and stained with Streptavidin Phycoerythrin on the Affymetrix Fluidics Station 450
|
| Scan protocol |
Microarrays were scanned using GeneChip® Scanner 3000 7G.
|
| Data processing |
The data were analyzed with the robust multichip analysis (RMA) algorithm using default analysis settings and global scaling as a normalization method Values are presented according to log2 RMA signal intensity
We defined the genes with a fold-change of more than 1.5 or less than 1.5 and a p-value of less than 0.05 as different expression genes, which were selected to undergo basic analysis
|
| |
|
| Submission date |
Aug 26, 2019 |
| Last update date |
Feb 04, 2020 |
| Contact name |
xu liu |
| E-mail(s) |
liu-xu@hotmail.com
|
| Organization name |
The Affiliated Hospital of Southwest Medical University
|
| Street address |
25th Taiping Street
|
| City |
Luzhou |
| ZIP/Postal code |
646000 |
| Country |
China |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE136331 |
Role of engrailed 2 (EN2) in esophageal squamous cell carcinoma |
|
