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| Status |
Public on Aug 22, 2019 |
| Title |
control1 |
| Sample type |
SRA |
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| Source name |
exosome
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| Organism |
Homo sapiens |
| Characteristics |
tissue: blood plasma
diagnosis: healthy
rna fraction: exosome
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the exosomes using TRIzol LS Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and quantified using the NanoDrop ND-1000.
RNA libraries were constructed by using NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., Massachusetts, USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).
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| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
control1
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| Data processing |
circRNA-seq reads were mapped to human genome by BWA-MEM software by default parameters except -T = 19
circRNAs were detected and annotated with CIRI software
Supplementary_files_format_and_content: excel format with Number of raw junction read
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| Submission date |
Aug 21, 2019 |
| Last update date |
Aug 22, 2019 |
| Contact name |
Zheng Zhang |
| E-mail(s) |
pfkzz1223@126.com
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| Organization name |
No.1 Hospital of China Medical University
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| Department |
Dermatology
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| Street address |
155N Nanjing Road, Heping district
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| City |
Shenyang‘ |
| ZIP/Postal code |
110001 |
| Country |
China |
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| Platform ID |
GPL20301 |
| Series (1) |
| GSE136113 |
circRNA-sequencing of blood plasma exosomes from cutaneous squamous cell carcinoma patients |
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| Relations |
| BioSample |
SAMN12614888 |
| SRA |
SRX6747974 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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