|
| Status |
Public on Aug 12, 2020 |
| Title |
T229_LA_Tumor tissue_Luminal A |
| Sample type |
RNA |
| |
|
| Source name |
Fresh frozen breast cancer tumor
|
| Organism |
Homo sapiens |
| Characteristics |
population: Mexico hispanic
pam50 molecular subtype: Luminal A
tissue: RNA from tumor tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We used AllPrep DNA/RNA Mini Kit
|
| Label |
biotin
|
| Label protocol |
WT Plus Reagent Kit were used to generate the biotinylated cRNA starting from 100ng of total RNA according to the manufacturer (Manual Target preparation for GeneChip Whole transcript (WT) expression arrays, Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Transcriptome Array 2.0 Arrays (Affymetrix)
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix). GeneChips were scanned using The Genechip Scanner 3000 7G (Affymetrix , Santa Clara, USA).
|
| Description |
SST-RMA expression value derived from Expression Console software
|
| Data processing |
Array signal intensitive were analyzed with the Affymetrix Expression Console using Signal Space Transformation-Robust Multichip Analysis (SST-RMA) for the background correction.
|
| |
|
| Submission date |
Jul 16, 2019 |
| Last update date |
Aug 12, 2020 |
| Contact name |
Alberto Cedro-Tanda |
| E-mail(s) |
acedro-tanda@comunidad.unam.mx
|
| Organization name |
INMEGEN
|
| Lab |
Cancer Genomics
|
| Street address |
Periferico sur #4809
|
| City |
CDMX |
| State/province |
CMDX |
| ZIP/Postal code |
14610 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE134359 |
Long noncoding RNA landscape in breast cancer [Mexico] |
|
