|
| Status |
Public on Aug 18, 2010 |
| Title |
6h High Interferon alpha MHH1 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC, 5 male healthy humans
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: natural killer cells
gender: male
treatment: recombinant IFNa-2b
dosage: 100 ng/ml
|
| Treatment protocol |
100 ng/ml recombinant IFNa-2b
|
| Growth protocol |
6h RPMI, 10% human AB serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 37°C, 5%CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000.
|
| Description |
Cultured for 6h with high dosage of Interferon alpha.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Apr 20, 2009 |
| Last update date |
Aug 21, 2009 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE15743 |
IFN alpha-induced gene expression in human NK cells |
|
