|
| Status |
Public on Apr 27, 2020 |
| Title |
Knockout 1week rep2 |
| Sample type |
SRA |
| |
|
| Source name |
heart tissue
|
| Organism |
Mus musculus |
| Characteristics |
genotype: LMNA knockout
timepoint: 1week
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mouse heart tissue were disected and RNA was harvested using RNeasy Fibrous Tissue Mini Kit. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Truseq Stranded Total RNA -RiboZero library was prepared
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
DEseq_1weekRNAseq_input.txt
|
| Data processing |
Pair-end raw fastq files ware aligned and assembled to mouse genome mm10 with STAR (v2.3.1d)
Quantified read counts from STAR were used for differential gene expression analysis using DEseq2 R package
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include raw counts for each Sample …
|
| |
|
| Submission date |
Jul 02, 2019 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Ying Ni |
| E-mail(s) |
niy@ccf.org
|
| Organization name |
Cleveland Clinic
|
| Department |
Quantitative Health Science
|
| Street address |
9500 Euclid Ave
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE133693 |
Myocardial RNA Sequence Analyses throughout the Course of Mouse Cardiac Laminopathy Identify Differential Expressions of the Key Genes for Cell Cycle Control and Mitochondrial Function |
|
| Relations |
| BioSample |
SAMN12186478 |
| SRA |
SRX6388019 |
