|
| Status |
Public on Oct 02, 2020 |
| Title |
HuTu13 cells siControl, biological rep1 [array] |
| Sample type |
RNA |
| |
|
| Source name |
HuTu13 cells (Mesenchymal) transfected with Control siRNA 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary Glioblastoma HuTu13 cells (Mesenchymal)
experimental condition: Control siRNA
|
| Treatment protocol |
Cells were transfected with siRNA by using RNAi MAX (ThermoFisher), according to the manufacturer's instructions.
|
| Growth protocol |
HuTu cells are cultured with DMEM-F12, 1% GLN, 1% PS, 10% BIT9500 (serum substitute), 25 ng/ml human EGF, 25 ng/ml human bFGF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the BioArrayTM HighYieldTM RNA Transcript Labeling Kit (ENZO Biochem, New York, NY) according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Standard Affymetrix scanning procedures on a GCS3000 7G Affymetrix scanner operated by GCOS, GeneChip Operating Software.
|
| Description |
13Co_A
Gene expression data from control HuTu13 cells.
Transfected with Control siRNA.
|
| Data processing |
RMA of .CEL files using Bioconductor affy library and Brainarray custom CDF HGU133Plus2_Hs_ENTREZG_v21. In RMA, PM values have been background adjusted, normalized using quantile normalization, and expression measure calculated using median polish summarization.
|
| |
|
| Submission date |
Jun 27, 2019 |
| Last update date |
Oct 03, 2020 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
|
| Phone |
+39-049-827-6108
|
| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23270 |
| Series (2) |
| GSE133470 |
Gene expression of primary Glioblastoma cell lines |
| GSE133471 |
Transcriptional effects of YAP/TAZ depletion in astrocytes, gliomaspheres, and glioblastoma cell lines |
|
