|
| Status |
Public on Aug 08, 2019 |
| Title |
SF295_GFP_sg1_A |
| Sample type |
SRA |
| |
|
| Source name |
SF295_GFP_sg1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SF295
genotype/variation: GFP_sg1
|
| Treatment protocol |
Cells infected with CRISPR vector were also selected with puromycin.
|
| Growth protocol |
RPMI with 10% FBS and 1% penicillin-streptomycin-glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Miniprep Kit (Qiagen)
KAPA mRNA HyperPrep Kit (Roche) for RNAseq
mRNA capture using magnetic oligo-dT beads
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
20180319_SF295GFPsg1-A_SC5356_S9
|
| Data processing |
Alignment: STAR 2.6.1d
Transcript quantification: RSEM 1.3.1
Genome_build: GRCh38
Supplementary_files_format_and_content: transcript quantification; tab-delimited text files include count, TPM values
|
| |
|
| Submission date |
Jun 25, 2019 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Steven Corsello |
| Organization name |
Broad Institute
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE133285 |
Transcriptional profiling of SF295 cells following MTF1 knockout by CRISPR/Cas9 |
| GSE133299 |
Discovering the anti-cancer potential of non-oncology drugs by systematic PRISM profiling |
|
| Relations |
| BioSample |
SAMN12136633 |
| SRA |
SRX6366225 |
