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Sample GSM38354 Query DataSets for GSM38354
Status Public on Oct 04, 2005
Title 10.Koth_60_HS.CEL
Sample type RNA
 
Source name isolated alveolar macrophages
Organism Homo sapiens
Characteristics status: Smoker
Extracted molecule total RNA
Extraction protocol RNA was extracted from mouse and human alveolar macrophages and DNase-treated using the RNeasy RNA extraction kit (Qiagen). RNA concentration and quality were assessed using the Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA).
Label protocol Labeling was performed at the UCSF core facility (cat.ucsf.edu) as per Affymetrix's standard labeling protocol for this chip type (www.affymetrix.com)
 
Hybridization protocol Samples were hybridized to Affymetrix Human Genome U133 Plus 2.0 GeneChips. RNA was ampli?ed by performing two rounds of in vitro transcription (Ambion Message Amp aRNA kit no. 1750).
Scan protocol Scanning was performed at the UCSF core facility (cat.ucsf.edu) as per Affymetrix's standard scanning protocol for this chip type (www.affymetrix.com)
Description Total RNA from freshly isolated macrophages
Data processing Bioconductor was used for quality control (affyPLM) and preprocessing (RMA).
 
Submission date Jan 04, 2005
Last update date Aug 28, 2018
Contact name Michael E. Salazar
Phone (415) 514-4371
URL http://arrays.ucsf.edu
Organization name University of California, San Francisco
Department
Lab Functional Genomics Core Laboratories
Street address 1550 Fourth Street, RM 545
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL570
Series (1)
GSE2125 isolated alveolar macrophages
Relations
Reanalyzed by GSE46903
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA normalized intensity

Data table
ID_REF VALUE
1007_s_at 7.266743
1053_at 7.583722
117_at 7.092117
121_at 10.521322
1255_g_at 4.817926
1294_at 8.735746
1316_at 7.04046
1320_at 6.511891
1405_i_at 4.339716
1431_at 4.886472
1438_at 6.336966
1487_at 8.00987
1494_f_at 7.02644
1552256_a_at 8.083462
1552257_a_at 9.351841
1552258_at 7.172738
1552261_at 6.626305
1552263_at 9.498443
1552264_a_at 8.673791
1552266_at 4.858038

Total number of rows: 54675

Table truncated, full table size 1056 Kbytes.




Supplementary file Size Download File type/resource
GSM38354.CEL.gz 8.6 Mb (ftp)(http) CEL

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