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| Status |
Public on Jun 01, 2019 |
| Title |
DV90_NC |
| Sample type |
SRA |
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| Source name |
DV90_NC
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| Organism |
Homo sapiens |
| Characteristics |
cell line: DV90
genotype/variation: control
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Cellular RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using mRNA-seq protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
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| Description |
siNC_R1
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| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg38 whole genome using Hisat2
mRNA and lncRNA annotated in NCBI were quantified using featureCounts
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
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| Submission date |
May 31, 2019 |
| Last update date |
Jun 06, 2019 |
| Contact name |
Shenglin Huang |
| Organization name |
Fudan University
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| Street address |
270 Dongan
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| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platform ID |
GPL21290 |
| Series (1) |
| GSE132011 |
RNA-seq of DV90 cells with REG4 siRNA |
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| Relations |
| BioSample |
SAMN11897708 |
| SRA |
SRX5938059 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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