|
| Status |
Public on Mar 20, 2009 |
| Title |
Hues 12 |
| Sample type |
RNA |
| |
|
| Source name |
undifferentiated human ES cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: undifferentiated human ES cells
development stage: embryonic
|
| Treatment protocol |
Chen et al., Cell Stem Cell 4, February 6, 2009, pp103-106
|
| Growth protocol |
Standard human embryonic stem cell media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy Plus Mini Kit Qiagen
|
| Label |
biotin
|
| Label protocol |
according to Illumina protocols
|
| |
|
| Hybridization protocol |
Hybridization according to Illumina protocols on Illumina humanRef-8 v3 BeadArray
|
| Scan protocol |
according to Illumina protocols
|
| Description |
Chip # 4256425034; Array B
|
| Data processing |
Variance stabilization before normalization using the vst function in the illumina package (http://www.bioconductor.org/packages/bioc/vignettes/lumi/inst/doc/lumi.pdf) Quantile normalization, due to normalization, there is no p value.
|
| |
|
| Submission date |
Mar 19, 2009 |
| Last update date |
Mar 19, 2009 |
| Contact name |
Dieter Egli |
| E-mail(s) |
degli@nyscf.org
|
| Phone |
617 4968659
|
| Organization name |
The New York Stem Cell Foundation Research Institute
|
| Department |
NYSCF
|
| Lab |
Egli
|
| Street address |
3960 Broadway
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE15294 |
Gene expression of genetically related and unrelated human embryonic stem cell lines |
|
