|
| Status |
Public on Mar 08, 2010 |
| Title |
Human hepatitis C virus liver biopsy sample 25 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from human hepatitis C virus liver biopsy sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue sample: HCV positive
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol procedure (Invitrogen)
|
| Label |
Cy3
|
| Label protocol |
Direct labeling with Cy3-pCp. See Agilent Technologies website for miRNA labeling protocol: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=50500
|
| |
|
| Hybridization protocol |
See Agilent Technologies website for miRNA microarray hybridization and wash protocol: http://www.chem.agilent.com/scripts/LiteraturePDF.asp?iWHID=50500
|
| Scan protocol |
Scanned on an Agilent G2505B Scanner
|
| Description |
Hepatitis C virus positive (HCV+) and negative (HCV-) tissue samples from percutaneous core needle biopsy specimens from liver transplantation patients were obtained according to clinical protocols at the University of Washington Medical Center.
|
| Data processing |
Agilent Feature Extraction v9.5.3, default miRNA parameters. The data table below represents all replicate probes per miRNA. The VALUE column reports the combined results from all replicate probes for a given miRNA (i.e., the same VALUE is reported multiple times for each replicate probe of a given miRNA).
|
| |
|
| Submission date |
Mar 18, 2009 |
| Last update date |
Mar 08, 2010 |
| Contact name |
Xinxia Peng |
| E-mail(s) |
xinxiap@u.washington.edu
|
| Organization name |
University of Washington
|
| Department |
Microbiology
|
| Lab |
Katze
|
| Street address |
Rosen Building, 960 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6955 |
| Series (2) |
| GSE15288 |
microRNA expression in human hepatitis c virus (HCV) liver biopsy samples |
| GSE15387 |
microRNA and mRNA expression in human hepatitis C Virus |
|
