|
Status |
Public on Mar 11, 2009 |
Title |
High Early-Life SES Subject # 770 |
Sample type |
RNA |
|
|
Source name |
PBMC
|
Organism |
Homo sapiens |
Characteristics |
socio-economic status: High Early-Life SES cell type: PBMC
|
Treatment protocol |
Leukocytes were collected by antecubtial venipuncture and PBMC were then isolated by density-gradient centrifugation
|
Growth protocol |
n/a
|
Extracted molecule |
total RNA |
Extraction protocol |
PBMC were then homogenized in QiaShredder Spin Columns (Qiagen) and the lysates were frozen at -80° C until RNA was extracted using AllPrep DNA/RNA kits (Qiagen; Mississauga, ON).
|
Label |
biotin
|
Label protocol |
Used the Illumina TotalPrep RNA Amplification Kit following manufacturer's instructions
|
|
|
Hybridization protocol |
RNA was hybridized to Illumina HumanRef-8 v3.0 Expression Beadchips following manufacturer's instructions
|
Scan protocol |
Chips were scanned on an Illumina Beadstation 500 at the McGill University and Génome Québec Innovation Centre.
|
Description |
N/A
|
Data processing |
None - these are raw hybridization intensities, averaged across the 30 bead replicates, provided by Illumina Beadstudio
|
|
|
Submission date |
Mar 10, 2009 |
Last update date |
Mar 10, 2009 |
Contact name |
Gregory E Miller |
E-mail(s) |
gemiller@psych.ubc.ca
|
Organization name |
UBC
|
Department |
Psychology
|
Street address |
2136 W Mall
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V6L1M4 |
Country |
Canada |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE15180 |
The Biological Residue of Low Early-Life Social Class |
|