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Sample GSM3731229 Query DataSets for GSM3731229
Status Public on Apr 20, 2019
Title 2-tag species mixing
Sample type SRA
Source name E13.5 mouse embryonic fibroblasts, HEK293T cell line
Organisms Homo sapiens; Mus musculus
Characteristics mouse cell type: E13.5 mouse embryonic fibroblasts
human cell line: HEK293T
Extracted molecule total RNA
Extraction protocol 10x Genomics Chromium single cell preparation.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Description 2tag
polyA RNA
Cells were encapsulated and library prepared using the 10X Chromium instrument with the Single Cell 3' Reagent Kits (v2 chemistry). BAM files and Gene-barcode matrices were generated using 10X Genomics Cellranger version 2(?).
Data processing Generation of fastq files via
Alignment to mm10 or hg19 genome build via
Genome_build: mm10 or hg19
Supplementary_files_format_and_content: barcodes.tsv, genes.tsv, matrix.mtx
Submission date Apr 19, 2019
Last update date Apr 20, 2019
Contact name Samantha Morris
Phone 3147478618
Organization name Washington University in St Louis
Street address Department of Developmental Biology, Washington University School of
City St Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
Platform ID GPL22245
Series (1)
GSE130065 CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics
BioSample SAMN11466694
SRA SRX5712375

Supplementary file Size Download File type/resource
GSM3731229_2tag.ct.classification.txt.gz 84.3 Kb (ftp)(http) TXT
GSM3731229_2tag.gem.classification.csv.gz 166.6 Kb (ftp)(http) CSV
GSM3731229_2tag.hg19.barcodes.tsv.gz 40.2 Kb (ftp)(http) TSV
GSM3731229_2tag.hg19.genes.tsv.gz 273.7 Kb (ftp)(http) TSV
GSM3731229_2tag.hg19.matrix.mtx.gz 32.6 Mb (ftp)(http) MTX
GSM3731229_2tag.mm10.barcodes.tsv.gz 34.2 Kb (ftp)(http) TSV
GSM3731229_2tag.mm10.genes.tsv.gz 228.5 Kb (ftp)(http) TSV
GSM3731229_2tag.mm10.matrix.mtx.txt.gz 23.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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