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Sample GSM372115 Query DataSets for GSM372115
Status Public on Feb 17, 2010
Title MSf 6
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics disease state: multiple sclerosis free
cell type: PBMC
age: 20 years
gender: F
Treatment protocol Peripheral blood mononuclear cells (PBMC) were separated on ficoll-hypaque gradient
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the TRIzol Reagent (Invitrogen, Carlsbad, CA)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA
Hybridization protocol cDNA was synthesized, labeled, hybridized to HG-U133A-2 array containing 22,215 gene-transcripts according to Affymetrix (Inc, Santa Clara, CA) protocol
Scan protocol Hewlett Packard, GeneArray-TM scanner G2500A
Description working set74
Data processing Data analysis was performed by Partek Genomics Solution software ( ). Expression values were computed from raw CEL files by applying the Robust Multi-Chip Average (RMA) background correction algorithm. The RMA correction included: 1) values background correction; 2) quantile normalization; 3) log2 transformation; 4) median polish summarization. In order to avoid the noise caused by variable set effects we normalized each set to pre-saved distribution pattern of a well balanced set used as a reference distribution.
Submission date Feb 18, 2009
Last update date Feb 19, 2009
Contact name Michael Gurevich
Organization name Sheba Medical Center
Department Multiple Sclerosis Center
Lab Neurogenomics and Neuroimmunology
Street address Tel Hashomer
City Ramat Gan
ZIP/Postal code 52621
Country Israel
Platform ID GPL571
Series (1)
GSE14895 The trait of MS: Altered transcription regulation of nuclear receptors networks operate in the pre-disease state

Data table header descriptions
VALUE RMA signal

Data table
1007_s_at 3.65972
1053_at 2.11278
117_at 2.65946
121_at 6.785
1255_g_at 2.0597
1294_at 2.95909
1316_at 3.15508
1320_at 2.65772
1405_i_at 1.96356
1431_at 2.08843
1438_at 2.87197
1487_at 2.60224
1494_f_at 3.54771
1598_g_at 5.21994
160020_at 5.07068
1729_at 3.69604
1773_at 2.29035
177_at 2.18837
179_at 6.98748
1861_at 2.33924

Total number of rows: 22215

Table truncated, full table size 408 Kbytes.

Supplementary file Size Download File type/resource
GSM372115.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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