|
| Status |
Public on Oct 01, 2019 |
| Title |
p1703_5_Donor 3 ctrl |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Control
donor: Donor 3
tissue: blood
cell type: monocyte
|
| Treatment protocol |
Monocytes were incubated for 48 h with 150 μg/ml factor H or medium only at 2.5 mln cells in 2.5 ml RPMI medium
|
| Growth protocol |
PBMCs from peripheral blood were isolated by density gradient centrifugation over LymphoPrep, followed by positive selection of monocytes with CD14 magnetic microbeads
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNEasy kit as per the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manua, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Clariom D microarray.
|
| Scan protocol |
Arrays were scanned on a GeneChip 3000 scanner.
|
| Data processing |
Basic Affymetrix chip and Experimental Quality Analyses were performed using the Expression Console Software v1.4.1.46. Probe summarization and Data Normalization was done using RMA.
|
| |
|
| Submission date |
Apr 11, 2019 |
| Last update date |
Oct 02, 2019 |
| Contact name |
Petter Storm |
| E-mail(s) |
petter.storm@med.lu.se
|
| Phone |
+46-40 39 12 27
|
| Organization name |
Lund University
|
| Department |
Developmental and Regenerative Neurobiology
|
| Street address |
Sölvegatan 19, BMC A11
|
| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE129670 |
Expression data from monocytes treated with complement factor H |
|
