|
| Status |
Public on Oct 25, 2011 |
| Title |
HT29 parental control(1) |
| Sample type |
RNA |
| |
|
| Source name |
colon cancer cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HT29, colon cancer cells
|
| Growth protocol |
the medium for this cell line is dmem. to make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
atmosphere: air, 95%; carbon dioxide (CO2), 5%
temperature: 37.0°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted by RNeasy mini kit (Cat. 74106; Qiagen, Hilden, Germany), and 100 ng to 1 ug of total RNA were subjected into reverse transcription using the SuperScriptTM III Reverse transcriptase kit (Invitrogen) as directed by the manufacturer.
|
| Label |
Biotin
|
| Label protocol |
standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
HT29, colon cancer cells
|
| Data processing |
RMA through R based affy module
|
| |
|
| Submission date |
Feb 10, 2009 |
| Last update date |
Oct 25, 2011 |
| Contact name |
Wei-Lun Hwang |
| Organization name |
National Yang Ming Chiao Tung University
|
| Department |
Department of Biotechnology and Laboratory Science in Medicine
|
| Lab |
WLH LAB
|
| Street address |
No.155, Sec.2, Linong Street
|
| City |
Taipei |
| ZIP/Postal code |
11221 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14773 |
Roles of EMT regulator in colon cancer |
|
