|
| Status |
Public on Mar 01, 2022 |
| Title |
MDA-MB-231_shC_2 |
| Sample type |
SRA |
| |
|
| Source name |
MDA-MB-231
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
genotype: control
|
| Growth protocol |
MDA-MB-231 cells were cultured in Leibovitz's L-15 Medium supplemented with 10% FBS and penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were extracted according to RNeasy plus kit (#74104, Qiagen).
Libraries were prepared according to the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Illumina bcl2fastq version 2.19 were used to the basecalling.
Reads was aligned to hg38 by star 2.5.3a with default parameters
Read count was summarized with HTSeq 0.8.0
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Mar 18, 2019 |
| Last update date |
Mar 01, 2022 |
| Contact name |
Liu Quentin |
| E-mail(s) |
18301246519@163.com
|
| Organization name |
DA LIAN MEDIACAL UNIVERSITY
|
| Street address |
Lvshunkou District, Dalian, Liaoning
|
| City |
DA LIAN |
| ZIP/Postal code |
116044 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE128428 |
Identification of genes are regulated by AURKA at transcription level |
|
| Relations |
| BioSample |
SAMN11158508 |
| SRA |
SRX5534185 |
