|
| Status |
Public on Apr 01, 2021 |
| Title |
HCT83-3LM PDO |
| Sample type |
RNA |
| |
|
| Source name |
PDO established from surgical specmen HCT83-3LM
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colorectal cancer
pdo derived from: metastasis tumor
|
| Treatment protocol |
Collected PDOs were directly used for RNA isolation.
|
| Growth protocol |
PDOs were collected and matrigel were removed using cell recovery solution.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using an RNeasy Micro Kit (Qiagen). RNA quality was validated using an Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA ( GeneChip WT PLUS Reagent Kit (Affymetrix)).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.2 ug of ss-cDNA were hybridized for 16 hr at 45C on GeneChip Human transcriptome Array 2. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
gene expression data of PDO derived from metastasis tumor
|
| Data processing |
The data were analyzed with Transcriptome analysis console 4.0 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Mar 13, 2019 |
| Last update date |
Apr 02, 2021 |
| Contact name |
Ryoji Yao |
| E-mail(s) |
ryao@jfcr.or.jp
|
| Organization name |
The JFCR-Cancer Institute
|
| Street address |
3-8-31 Ariake
|
| City |
Koto-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE128213 |
Expression data from CRC patient-derived organoids |
|
