|
| Status |
Public on Jan 29, 2009 |
| Title |
MCF7_CRY2KO_Rep1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
MCF7 Negative Control siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
MCF7 cells transfected with a negative control siRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy mini kit with on-column DNAse digestion
|
| Label |
Cy3
|
| Label protocol |
mRNA converted to Cyanine 3-labeled cRNA using Agilent's Quick Amp Labeling Kit
|
| |
|
| Channel 2 |
| Source name |
MCF7 CRY2 siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
MCF7 cells transfected with a CRY2-targeting siRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy mini kit with on-column DNAse digestion
|
| Label |
Cy5
|
| Label protocol |
mRNA converted to Cyanine 5-labeled cRNA using Agilent's Quick Amp Labeling Kit
|
| |
|
| |
| Hybridization protocol |
Agilent's Gene Expression Hybridization Kit followed by Agilent's Gene Expression Wash Buffer Kit
|
| Scan protocol |
Agilent’s Microarray Scanner with SureScan technology
|
| Description |
All protocols performed by an Agilent-certified service provider
|
| Data processing |
Agilent Feature Extraction Software, data were analyzed using the Rosetta Luminator gene expression data analysis system. Value = log10(intensity2/intensity1).
|
| |
|
| Submission date |
Jan 28, 2009 |
| Last update date |
Jan 28, 2009 |
| Contact name |
Aaron Ezra Hoffman |
| E-mail(s) |
aaron.hoffman@yale.edu
|
| Organization name |
Yale School of Medicine
|
| Department |
LEPH
|
| Lab |
702
|
| Street address |
60 College St.
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
|
