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Status |
Public on Jun 22, 2020 |
Title |
Biopsy_2hrs.reperfusion_patient_41 |
Sample type |
RNA |
|
|
Source name |
Biopsy_2hrs.reperfusion
|
Organism |
Homo sapiens |
Characteristics |
paired samples: patient_41 timepoint: 2HR_Reperf tissue: peripheral lung biopsy
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from snap frozen samples using the RNeasy Mini Kit (Qiagen; Hilden, Germany). RNA quality was assessed using Nanodrop spectrophotometer (VWR; Radnor, PA) and Bioanalyzer (Agilent; Santa Clara, CA)
|
Label |
biotin
|
Label protocol |
According to manufacturer guidelines
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|
|
Hybridization protocol |
According to manufacturer guidelines
|
Scan protocol |
According to manufacturer guidelines
|
Description |
796.rep2h
|
Data processing |
Affymetrix CEL files were imported and processed using RMA in R version 3.5.1 with the affy package. We used an alternative CDF probset description file from Brainarray (version 20) at the University of Michigan (Nucleic Acid Research 33 (20), e175; http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/genomic_curated_CDF.asp): hgu133plus2hsentrezgcdf_20.0.0.zip
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|
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Submission date |
Feb 25, 2019 |
Last update date |
Jun 24, 2020 |
Contact name |
Ricardo Zamel |
Organization name |
University Health Network
|
Street address |
101 College St.
|
City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5S 1L7 |
Country |
Canada |
|
|
Platform ID |
GPL22321 |
Series (2) |
GSE127003 |
Expression of Human Lung Before and After Transplantation |
GSE127243 |
Potential Therapeutic Targets for Donor Lung Repair During Human Ex Vivo Lung Perfusion Prior to Transplantation |
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