|
| Status |
Public on Feb 12, 2019 |
| Title |
biofluid_ascites_GC_19501344F |
| Sample type |
RNA |
| |
|
| Source name |
biofluid_ascites_GC_19501344F
|
| Organism |
Homo sapiens |
| Characteristics |
sample: ascites
gender: male
age (years): 69
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Exosome from biofluid was precipitated using miRCURY Exosome Isolation Kit (Exiqon). Exosomal RNA was isolated using Trizol LS Reagent (Molecular Research Center, Cincinnati, OH, USA), according to the manufacturer’s instructions. Total RNA was quantified by absorbance at 260 nm and the integrity of small RNA was checked by Bioanalyzer2100 (Agilent, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
Cyanine 3-labeled cRNA (complementary RNA) was generated from Agilent’s Low RNA Input Linear Amplification kit with 500ng total RNA. Labeled cRNA was also measured using Nanodrop spectrophotometer.
|
| |
|
| Hybridization protocol |
Labeled cRNA was applied microarray (Agilent technologies, 8x60K) using Agilent’s Gene Expression Hybridization Kit.
|
| Scan protocol |
The microarray chip was scanned using Agilent’s DNA microarray scanner and the raw signal density was acquired from feature Extraction software.
|
| Description |
gastric cancer-associated malignant ascites containing adenocarcinoma cells
|
| Data processing |
The differential gene expression was classified by Benjamini-Hochberg's false discovery ratio method. GeneSpring GX12 software. Gene ontology and KEGG pathway for the differential expressed genes was analyzed using GeneSpring GX 12 (Agilent Technologies, San Carlos, CA) and DAVID Bioinformatics tools (National Institute of Allergy and Infectious Diseases, NIH).
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| |
|
| Submission date |
Feb 11, 2019 |
| Last update date |
Feb 12, 2019 |
| Contact name |
Hye Sook Han |
| E-mail(s) |
sook3529@hanmail.net
|
| Organization name |
College of Medicine, Chungbuk National University
|
| Street address |
Chungdae-ro 1, Seowon-gu
|
| City |
Cheongju |
| ZIP/Postal code |
28644 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL18402 |
| Series (1) |
| GSE126399 |
Ascites-derived circulating microRNAs as potential diagnostic biomarkers of gastric cancer-associated malignant ascites |
|
