|
| Status |
Public on Nov 25, 2019 |
| Title |
Vehicle rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse liver tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
treatment: DMSO
age: 8 weeks
tissue: Liver
|
| Treatment protocol |
The mice were treated i.p. with pregnenolone-16-α-carbonitrile (PCN, 50mg/kg dissolved in DMSO-corn oil 1:3) or vehicle (DMSO-corn oil 1:3) once daily for four days. The mice were fasted for four hours and then administered glucose (2g/kg) by oral gavage or further kept on fasting. The mice were sacrificed 1 hour after glucose administration. The livers were collected and frozen in liquid nitrogen and used for total RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from liver tissue was isolated as described previously by Trizol reagent
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina® Total PrepT RNA Ampification kit (Life Technologies) for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
SAMPLE 160
|
| Data processing |
The data was log2-transformed, normalized with shift to 75 percentile and baseline transformed to median of all samples in GeneSpring 14.5 Software
|
| |
|
| Submission date |
Jan 25, 2019 |
| Last update date |
Nov 25, 2019 |
| Contact name |
Jaana Rysae |
| E-mail(s) |
jaana.rysa@gmail.com
|
| Organization name |
University of Eastern Finland
|
| Department |
School of Pharmacy
|
| Street address |
Yliopistonranta 1C
|
| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
| |
|
| Platform ID |
GPL21278 |
| Series (1) |
| GSE125695 |
The effect pregnane receptor X (PXR) agonist PCN on hepatic gene expression in mouse liver with and without glucose |
|
