|
| Status |
Public on Feb 15, 2019 |
| Title |
B2B1r3 |
| Sample type |
SRA |
| |
|
| Source name |
B cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Peritoneal cavity
cell type: B2B1
genetic background: C57BL/6 (backcrossed)
strain: B1-8VH12/Vk4
|
| Treatment protocol |
The BCR switch in the B2toB1 cells was induced ~30 days prior to isolation
|
| Growth protocol |
Cells were isolated from spleens and peritoneal cavities
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated using the RNEasy MICRO kit (Qiagen)
The libraries were generated using the TruSeq RNA Sample Kit v2-SetA (Illumina) according to the manufacturer’s protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Illumina CASAVA (v1.8.2) software was used for basecalling.
RNAseq reads were mapped to the mRNAs of mm10 using kallisto (kallisto -b 100 -t 2 -l 200 -s 75 --single [reads])
tpms were summed up per gene
Genome_build: mm10
Supplementary_files_format_and_content: tsv file with tpm
|
| |
|
| Submission date |
Jan 08, 2019 |
| Last update date |
Feb 15, 2019 |
| Contact name |
Robin Graf |
| E-mail(s) |
robin.graf@mdc-berlin.de
|
| Phone |
+493094062396
|
| Organization name |
Max Delbrueck Center for Molecular Medicine
|
| Department |
Immune Regulation and Cancer
|
| Lab |
Klaus Rajewsky
|
| Street address |
Robert Rössle Str. 10
|
| City |
Berlin |
| ZIP/Postal code |
13092 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN10715437 |
| SRA |
SRX5228518 |
