|
Status |
Public on Jun 01, 2011 |
Title |
Training65 |
Sample type |
RNA |
|
|
Source name |
Pretreatment sample_Training set
|
Organism |
Homo sapiens |
Characteristics |
Pretreatment endoscopic biopsy samples collected from CF-treated metastatic gastric cancer patients
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from tumor-rich area using Trizol, according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
|
|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix G3000 GeneArray Scanner
|
Description |
Training set to identify the signature for the intrinsic resistance to CF
|
Data processing |
The data were processed with R (v 2.6.1) and BRB-ArrayTools (v 3.6).
|
|
|
Submission date |
Dec 24, 2008 |
Last update date |
Jun 01, 2011 |
Contact name |
Jeffrey E Green |
E-mail(s) |
JEGreen@mail.nih.gov
|
Phone |
3014355193
|
Fax |
3014968709
|
Organization name |
NCI
|
Street address |
Rm 4054, 37 Convent Drive
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (2) |
GSE14208 |
Clinical response of metastatic gastric cancer patients to cisplatin and fluorouracil (CF) combination chemotherapy |
GSE14210 |
Expression data from human endoscopic biopsy samples |
|