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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 26, 2019 |
| Title |
IL-7+IL-23 |
| Sample type |
SRA |
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| Source name |
IL-7+IL-23 sitmulated memory-like CD4+ T cell
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype/variation: WT
cell type: mouse memory-like CD4 T cell
treatment: IL-7+IL-23
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
723_FPKM
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| Data processing |
HCS 3.3.52 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using fastx_trimmer), then mapped to mm10 whole genome using Tophat
Read count extraction and nomalization were performed using edgeR.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include read count values for each Sample
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| Submission date |
Dec 18, 2018 |
| Last update date |
Feb 26, 2019 |
| Contact name |
Hong-Gyun Lee |
| E-mail(s) |
chriseraser@gmail.com
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| Phone |
82-10-9488-1505
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| Organization name |
Hanyang University
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| Street address |
#527, Natural Science building, Hanyang Univ, Wangsimni-ro 222, Seondong-gu
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| City |
Seoul |
| ZIP/Postal code |
04763 |
| Country |
South Korea |
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| Platform ID |
GPL21103 |
| Series (1) |
| GSE124045 |
RNA-sequencing of bystander-activated memory-like CD4+ T cells |
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| Relations |
| BioSample |
SAMN10611390 |
| SRA |
SRX5160560 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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