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Status |
Public on Dec 18, 2008 |
Title |
H929 myeloma cells-treated_DMSO_4h |
Sample type |
RNA |
|
|
Source name |
H929 myeloma cells-treated_DMSO_4h
|
Organism |
Homo sapiens |
Characteristics |
tissue: multiple myeloma cell line t(4;14)
|
Treatment protocol |
Cells were treated with DMSO 0.1% (+/- pristimerin 500nM dissolved in DMSO) for 4h at 37oC
|
Growth protocol |
Myeloma cell lines were cultured in RPMI 10% FBS at 37oC in 5%CO2
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16h at 45 degrees Celsius onto Affymetrix GeneChip arrays with rotisserie mixing at 60rpm. GeneChips were washed and stained with streptavidin-phycoerythrin.
|
Scan protocol |
GeneChips were scanned using the GeneChip system confocal scanner.
|
Description |
H929 myeloma cells were treated with DMSO 0.1% for 4h at 37oC and total RNA was prepared using Trizol
|
Data processing |
The data were PLIER16 transformed using Affymetrix Expression Console and Excel and paired with detection calls generated from Affymetrix Expression ConsoleMAS5 analysis using default analysis settings
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Submission date |
Dec 17, 2008 |
Last update date |
Aug 28, 2018 |
Contact name |
rodger tiedemann |
E-mail(s) |
tiedemann.rodger@mayo.edu
|
Organization name |
Mayo Clinic
|
Department |
Division of Hematology Oncology
|
Street address |
13400 E Sahuaro Dr
|
City |
Scottsdale |
State/province |
AZ |
ZIP/Postal code |
85259 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE14011 |
Expression data from myeloma cell lines treated with 500 nM pristimerin or DMSO vehicle alone for 4 hours |
|
Relations |
Reanalyzed by |
GSE119087 |