|
| Status |
Public on Jul 15, 2019 |
| Title |
ExE mutant replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
mutant_extraembryonic ectoderm
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
developmental stage: E6.5 embryo
genotype/variation: Esrrb mutant
tissue: Extraembryonic ectoderm
|
| Treatment protocol |
Post-implantation embryos were dissected from the uteri of pregnant females at E6.5. Extraembryonic ectoderm was separated and pooled (~20-25 per replicate)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol, and the quality of the RNA was assessed using a BioAnalyzer
|
| Label |
Biotin
|
| Label protocol |
100 ng of RNA from each pooled sample was used to generate cDNA, which was then fragmented and labeled using the Ambion WT Expression Kit according to the manufacturer's protocol
|
| |
|
| Hybridization protocol |
cDNA was then hybridized to Mouse 1.0 ST gene array v1 according to the manufacturer’s protocol
|
| Scan protocol |
Chip was scanned with an Affymetrix GeneChip Scanner 7G
|
| Description |
Esrrb_mutant_rep3
|
| Data processing |
The raw data was imported and normalized using the affy R package v.1.44.0
|
| |
|
| Submission date |
Dec 05, 2018 |
| Last update date |
Jul 17, 2019 |
| Contact name |
Eiichi Okamura |
| E-mail(s) |
eiokamu@gmail.com
|
| Organization name |
Shiga University of Medical Science
|
| Department |
Research Center for Animal Life Science
|
| Lab |
Department of Stem Cells and Human Disease Models
|
| Street address |
Seta, Tsukinowa-cho
|
| City |
Otsu |
| State/province |
Shiga |
| ZIP/Postal code |
520-2192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE123360 |
Esrrb function in the extraembryonic ectoderm is required for proper primordial germ cell specification [microarray] |
| GSE123363 |
Esrrb function is required for proper primordial germ cell development in presomite stage mouse embryos |
|
