|
| Status |
Public on Jun 27, 2020 |
| Title |
T352-2 |
| Sample type |
SRA |
| |
|
| Source name |
flesh cells
|
| Organism |
Prunus persica |
| Characteristics |
stored temperature(℃）: 35
stored times(day): 2
tissue: flesh cells
|
| Treatment protocol |
Postharvest peach fruit were pre-stored on 5℃ for 2 d, and divided into two random groups under 2 different temperatures of 25℃, 35℃
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extract total RNA from each sample,then use Ribo-Zero Magnetic Kit to delete rRNA.
RNA libraries were prepared using Directional RNA Library Prep Kit for Illumina. Libraries were sequenced on Illumina HiSeq 2500.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
rRNA-depleted RNA
|
| Data processing |
Data filtered of the raw data using FASTX-Toolkit tools(version 0.0.13.2) and tested the results using FastQC(version0.10.1).
Read mapped to the published genome of Prunus persica (verde et al 2013) using Bowtie2(version 2.1.0) and TopHat(version 2.0.10), Samtools(version 0.1.18),.
Fragments Per Kilobase of exon per Million Fragments Mapped(FPKM) were calculated using a series of Cufflinks software(version 2.1.1).
Genome_build: Prunus_persica_NCBIv2
Supplementary_files_format_and_content: csv files include FPKM values for each Sample ...
|
| |
|
| Submission date |
Nov 23, 2018 |
| Last update date |
Jun 27, 2020 |
| Contact name |
xiao wang |
| E-mail(s) |
xwang1234@163.com
|
| Organization name |
Huazhong Agricutural University
|
| Street address |
No.1 Shizishan street, Hongshan district, Wuhan City, Hubei Province
|
| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL15671 |
| Series (1) |
| GSE122868 |
Comparative transcriptomic analysis of peach fruits in common and high temperature conditions |
|
| Relations |
| BioSample |
SAMN10472859 |
| SRA |
SRX5058013 |
