|
| Status |
Public on Nov 24, 2018 |
| Title |
F1-3 |
| Sample type |
SRA |
| |
|
| Source name |
leaves
|
| Organism |
Zea mays |
| Characteristics |
developmental stage: seedlings
tissue: leaves
genotype: An'nong 591 F1
treatment: Heat treatment for 2 days
|
| Treatment protocol |
42 °C/35 °C (day/night) for two days
|
| Growth protocol |
Seedlings were grown in a greenhouse at 28 °C/23 °C (day/night) with a 16-h light/8-h dark photoperiod
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent,1 μg of total RNA with RNA Integrity Number (RIN) values above 7 was used for library construction
The cDNA library preparations were constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina®
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Raw reads were filtered to obtained clean reads
Clean reads were aligned to the reference genome with HISat software (v2.0.1)
Gene expression level was measured using HTSeq software (V0.6.1)
The significantly differentially expressed genes were identified using the DESeq R package (1.10.1) with fold changes ≥2 and FDR ≤ 0.05
Genome_build: maize B73 reference genome (RefGen_v3)
Supplementary_files_format_and_content: xls file include fpkm values for the 18 samples
|
| |
|
| Submission date |
Nov 23, 2018 |
| Last update date |
Nov 26, 2018 |
| Contact name |
Yang Zhao |
| E-mail(s) |
zhaoyang0521@163.com
|
| Organization name |
Anhui Agricultural University
|
| Street address |
changjiang road 130
|
| City |
Hefei |
| ZIP/Postal code |
230036 |
| Country |
China |
| |
|
| Platform ID |
GPL25842 |
| Series (1) |
| GSE122866 |
Transcriptome analysis of maize hybrid An’nong 591 and its parental lines under heat stress |
|
| Relations |
| BioSample |
SAMN10472528 |
| SRA |
SRX5057991 |
