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Sample GSM3487497 Query DataSets for GSM3487497
Status Public on Nov 24, 2018
Title CR-2
Sample type SRA
 
Source name leaves
Organism Zea mays
Characteristics developmental stage: seedlings
tissue: leaves
genotype: paternal line
treatment: control
Treatment protocol 42 °C/35 °C (day/night) for two days
Growth protocol Seedlings were grown in a greenhouse at 28 °C/23 °C (day/night) with a 16-h light/8-h dark photoperiod
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent,1 μg of total RNA with RNA Integrity Number (RIN) values above 7 was used for library construction
The cDNA library preparations were constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina®
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Raw reads were filtered to obtained clean reads
Clean reads were aligned to the reference genome with HISat software (v2.0.1)
Gene expression level was measured using HTSeq software (V0.6.1)
The significantly differentially expressed genes were identified using the DESeq R package (1.10.1) with fold changes ≥2 and FDR ≤ 0.05
Genome_build: maize B73 reference genome (RefGen_v3)
Supplementary_files_format_and_content: xls file include fpkm values for the 18 samples
 
Submission date Nov 23, 2018
Last update date Nov 26, 2018
Contact name Yang Zhao
E-mail(s) zhaoyang0521@163.com
Organization name Anhui Agricultural University
Street address changjiang road 130
City Hefei
ZIP/Postal code 230036
Country China
 
Platform ID GPL25842
Series (1)
GSE122866 Transcriptome analysis of maize hybrid An’nong 591 and its parental lines under heat stress
Relations
BioSample SAMN10472535
SRA SRX5057984

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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