|
| Status |
Public on Dec 06, 2008 |
| Title |
hESC-CM-3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
hESC-CM-3
|
| Organism |
Homo sapiens |
| Characteristics |
Cardiomyocytes that were differentiated then purified from hESCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated as described previously in Trizol (Invitrogen) followed by purification over a Qiagen RNeasy column (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Using Low RNA Input Fluorescent Linear Amplification Kits (Agilent Technologies, Santa Clara, CA, USA), cDNA was reverse transcribed from each RNA sample, and cRNA was then transcribed and fluorescently labeled from each cDNA sample.
|
| |
|
| Channel 2 |
| Source name |
Pooled Reference
|
| Organism |
Homo sapiens |
| Characteristics |
Pooled RNA from undiffereniated and differentiated hESCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated as described previously in Trizol (Invitrogen) followed by purification over a Qiagen RNeasy column (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Using Low RNA Input Fluorescent Linear Amplification Kits (Agilent Technologies, Santa Clara, CA, USA), cDNA was reverse transcribed from each RNA sample, and cRNA was then transcribed and fluorescently labeled from each cDNA sample.
|
| |
|
| |
| Hybridization protocol |
825 ng of Cy3- and Cy5- labeled and amplified cRNA was mixed and fragmented according to the Agilent technology protocol. cRNA was hybridized to 4x44K whole human genome microarray slides from Agilent (Part G4112F) according to the manufacturer’s instructions. The hybridization was carried in a rotating hybridization chamber in the dark at 65°C for 17 h.
|
| Scan protocol |
The array was scanned using Agilent G2505B DNA microarray scanner.
|
| Description |
hESC-CM-3
|
| Data processing |
The image files were extracted using Agilent Feature Extraction software version 9.5.1 applying LOWESS background subtraction and dye-normalization.
|
| |
|
| Submission date |
Dec 04, 2008 |
| Last update date |
Dec 05, 2008 |
| Contact name |
Kitchener D. Wilson |
| E-mail(s) |
kitchwilson@stanford.edu
|
| Organization name |
Stanford University
|
| Street address |
S140 Grant Bldg
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE13834 |
Transcriptional Profiling of Human Embryonic Stem Cell-Derived Cardiomyocytes |
|
