|
| Status |
Public on Dec 02, 2009 |
| Title |
CRT knockout mouse embryonic stem cell, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Calreticulin knockout of mouse embryonic stem cell line
|
| Organism |
Mus musculus |
| Characteristics |
Strain: D3, mouse embryonic stem cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix 430 2.0 Mouse Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
| Description |
Gene expression data from murine embryonic stem cells with calreticulin deficiency
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Dec 03, 2008 |
| Last update date |
Dec 03, 2008 |
| Contact name |
Randolph Faustino |
| E-mail(s) |
faustino.randolph@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Street address |
200 First Street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE13805 |
Expression data from wild type and calreticulin deficient murine embryonic stem cells |
|
