|
| Status |
Public on Jul 15, 2020 |
| Title |
KW423Soy10 |
| Sample type |
SRA |
| |
|
| Source name |
normal mammary gland PND55
|
| Organism |
Mus musculus |
| Characteristics |
time of collection: postnatal day (PND) 55
strain: FVB
diet: 1% (10g/kg) isolated soy protein
|
| Treatment protocol |
mating mice were fed 20% ISP, 1% ISP, 20% casein or Teklad 2018 diets and mother's were maintained on this diet during lactation and pups were maintained on this diet after weaning and until the end of the study
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using mirVana miRNA Isolation Kit following the manufacturer's protocol
mRNA stranded library preparation (NEB) was performed by Genome Quebec Innovation Centre
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
reads trimmed from 3' end to have a phred score of at least 30
illumina sequencing adapaters removed from the reads and all reads required to have length of at least 32bp
trimming and clipping performed using Trimmomatic
Genome_build: GRCm38
|
| |
|
| Submission date |
Nov 07, 2018 |
| Last update date |
Jul 15, 2020 |
| Contact name |
Roger Moorehead |
| E-mail(s) |
rmoorehe@uoguelph.ca
|
| Organization name |
University of Guelph
|
| Department |
Biomedical Sciences
|
| Street address |
50 Stone Rd E
|
| City |
Guelph |
| ZIP/Postal code |
N1G2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE122271 |
RNA sequencing of PND55 mammary glands of mice fed 20% ISP, 1% ISP, 20% casein or Teklad 2018 |
| GSE122316 |
RNA sequencing of pubertal mammary glands and mammary tumors from MTB-IGFIR transgenic mice fed diets containing different levels of isolated soy protein |
|
| Relations |
| BioSample |
SAMN10392574 |
| SRA |
SRX4993013 |
