|
| Status |
Public on Jan 17, 2019 |
| Title |
PCNSL21 |
| Sample type |
RNA |
| |
|
| Source name |
PCNSL tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary central nervous system lymphomas
age: 31
gender: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isogen extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
|
| Description |
miRNA expression data from PCNSL
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 31, 2018 |
| Last update date |
Jan 17, 2019 |
| Contact name |
Ryuya Yamanaka |
| E-mail(s) |
ryaman@koto.kpu-m.ac.jp
|
| Organization name |
Kyoto Prefectural University of Medicine
|
| Lab |
Molecular target therapy for cancer
|
| Street address |
465 Kajii-cho, Kamigyoku
|
| City |
Kyoto |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE122011 |
miRNA expression data from primary central nervous system lymphomas (PCNSL) |
|
