|
| Status |
Public on Dec 23, 2009 |
| Title |
NP cells 3D before differentiation rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Neural progenitor cells without differentiation cultured in 3D polystyrene scaffolds
|
| Organism |
Homo sapiens |
| Characteristics |
Neural progenitor cells cultured in 3D scaffolds
|
| Treatment protocol |
Differentiation Medium was used to replace ordinary growth medium to induce differentiation. The composition of both media can be found in millipore standard protocol
|
| Growth protocol |
Human Progenitor cells were cultured as standard protocol from Millipore
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by RNAeasy mini kit (Qiagen) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The standard hybridization protocol from Affymetrix was followed
|
| Scan protocol |
Arrays were scanned at high resolution using an Affymetrix GeneChip Scanner 3000 located at Medical College of Georgia Microarray Core Facility (Augusta, GA)
|
| Description |
Gene expression data from cultured human neural progenitor cells
|
| Data processing |
The data were analyzed with Gene Expression Console using Affymetrix default Plier analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 24, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
William Kisaalita |
| E-mail(s) |
williamk@engr.uga.edu
|
| Phone |
7065420835
|
| Organization name |
University of Georgia
|
| Street address |
Dritmier Engineering Center
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30605 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13715 |
Expression data from Neural Progenitor cells cultured on 2D flat surfaces and in 3D scaffolds |
|
| Relations |
| Reanalyzed by |
GSE119087 |
