|
| Status |
Public on Nov 16, 2009 |
| Title |
Ho994 pre 2 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis muscle
|
| Organism |
Homo sapiens |
| Characteristics |
time of biopsy: Resting biopsy before 30 minutes of endurance exercise in the trained state.
|
| Treatment protocol |
Resting biopsies were taken from the vastus lateralis muscle using the Bergstroem technique.
Tissue was snap frozen in liquid nitrogen-cooled isopentane and kept frozen at -70 deg C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from 25 micrometer cryosections of the muscle biopsies using RNeasy kit (Qiagen).
2.5 micogram total RNA was reverse-transcribed using Superscript II (Invitrogen, Basel, Switzerland).
|
| Label |
32P
|
| Label protocol |
Standardized amounts of [alpha-32P-dCTP] were used per reaction. Radiolabelled cDNA target was purified from the reaction using the supplied cartridge (BD Biosciences).
|
| |
|
| Hybridization protocol |
Equal amounts (1-3 Mio cpm) of cDNA target were hybridized to individual cDNA array filters in Expresshyp (BD Biosciences, Allschwil, Switzerland) at 68ºC o/n
|
| Scan protocol |
Filters were washed and exposed to a phosphor imager for 10 days (Molecular Dynamics, Sunnyvale, USA).
|
| Description |
Samples corresponding to different time-points of recovery from exercise of one individual before or after training, respectively, were analyzed in parallel.
|
| Data processing |
Expression was quantified from raw signal intensities in relation to the background as estimated using the Grid Background Dots mode for 54 selected dots (AIDA Array Easy software, Raytest, Urdorf, Switzerland).
|
| |
|
| Submission date |
Nov 16, 2008 |
| Last update date |
May 13, 2009 |
| Contact name |
Martin Flück |
| E-mail(s) |
flueck@ana.unibe.ch
|
| Phone |
+41 31 631 4619
|
| Fax |
+41 31 631 3807
|
| Organization name |
University of Berne
|
| Department |
Department of Anatomy
|
| Lab |
Hoppeler
|
| Street address |
Bühlstrase 26
|
| City |
Berne 9 |
| State/province |
Bern |
| ZIP/Postal code |
3000 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1935 |
| Series (1) |
| GSE13623 |
Hypoxic endurance exercise in an untrained or six weeks trained state |
|
