|
| Status |
Public on Oct 09, 2020 |
| Title |
RNA-Testis_E135_Wt_Mm_R2 |
| Sample type |
SRA |
| |
|
| Source name |
Testis
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Wild type
age: Embryonic day 13.5
strain: CD1
|
| Treatment protocol |
Gonads were disected rapidly after the animal was sacrified and then preserved in RNA later until the extraction
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted from the gonads using the RNeasy kit from Qiagen
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
For all samples 50bp paired-end reads were mapped against the respective genome (talOcc4, mm9) using STAR v2.6.1d (Dobin et al., 2013) with the following options: --chimSegmentMin 10 alignIntronMin 20 outFilterMismatchNoverReadLmax 0.05 --outFilterMismatchNmax 10 --outFilterMultimapNmax 5.
Genome_build: talOcc4, mm9
|
| |
|
| Submission date |
Sep 27, 2018 |
| Last update date |
Oct 10, 2020 |
| Contact name |
Francisca Martinez Real |
| E-mail(s) |
martinez@molgen.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Street address |
Ihnestrasse 73
|
| City |
Berlin |
| ZIP/Postal code |
D-14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE120582 |
The mole genome reveals regulatory rearrangements associated with adaptive intersexuality (RNA-Seq) |
| GSE120589 |
The mole genome reveals regulatory rearrangements associated with adaptive intersexuality |
|
| Relations |
| BioSample |
SAMN10138977 |
| SRA |
SRX4746083 |
