NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3396175 Query DataSets for GSM3396175
Status Public on Sep 21, 2018
Title Bone marrow O
Sample type SRA
 
Source name Bone marrow
Organism Homo sapiens
Characteristics cell type: bone marrow cells
age: 50 years old
Treatment protocol No treatment
Growth protocol Using standard operating procedures, mononuclear cells from 20 healthy donors' bone marrow aspirates were isolated using Ficoll density gradient separation and cryopreserved in 90% FBS/ 10% DMSO for storage in 72 liquid nitrogen.
Extracted molecule polyA RNA
Extraction protocol scRNAseq was performed using 10X Genomics Single Cell 3’ Solution, version 2 according to manufacturer’s instructions (protocol rev A).
Droplet-based scRNAseq
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
 
Data processing Data were analyzed using Cell Ranger V2.0.0 (10X Genomics).
Genome_build: GRCh38
Supplementary_files_format_and_content: Cell Ranger generated one mtx file and two tsv files for gene ID and cell barcode ID.
 
Submission date Sep 19, 2018
Last update date Sep 24, 2018
Contact name Christopher Hourigan
E-mail(s) christopher.hourigan@nih.gov
Phone 3014510257
Organization name National Heart Lung and Blood Institute, NIH
Department Hematology Branch
Lab Myeloid Malignancies
Street address Room 10CRC 5-5130, 10 Center Drive
City Bethesda
State/province Maryland
ZIP/Postal code 20892
Country USA
 
Platform ID GPL21290
Series (2)
GSE120221 Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry [scRNA]
GSE120446 Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry
Relations
BioSample SAMN10095258
SRA SRX4720051

Supplementary file Size Download File type/resource
GSM3396175_barcodes_O.tsv.gz 20.3 Kb (ftp)(http) TSV
GSM3396175_genes_O.tsv.gz 258.6 Kb (ftp)(http) TSV
GSM3396175_matrix_O.mtx.gz 16.2 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap