|
| Status |
Public on Jul 21, 2018 |
| Title |
knockout mouse #6 - stimulated |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
activation conditions: activated with anti-CD3/anti-CD28 (1 µg/each)
strain: mixed B6/129
age: Young adult
cell type: Naïve (CD62Lhi) CD4+ T cell
|
| Growth protocol |
T cells were cultured at 0.2 million cells per well in 96 well flat bottomed plates in complete media. Plates were pre-coated with anti-CD3 and anti-CD28
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen RNA easy kits
|
| Label |
Cy3
|
| Label protocol |
Illumina TotalPrep-96 Amplification kit (Ambion) was used as per manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Mouse WG-6 v2.0 BeadChips were incubated at 58oC, with rotation speed 5 for 18 hours.
|
| Scan protocol |
BeadChips were scanned on the iScan (Illumina).
|
| Description |
KO biological replicate # 6-stim
|
| Data processing |
Data files were quanitified in GenomeStudio v 2010.1 (Illumina). Quantile normalization was performed using GeneSpring v12.1 (Agilent).
|
| |
|
| Submission date |
Jul 20, 2018 |
| Last update date |
Jul 21, 2018 |
| Contact name |
Shannon Dunn |
| E-mail(s) |
Shannon.Dunn@unityhealth.to
|
| Organization name |
St. Michael's Hospital
|
| Department |
Keenan Research Centre for Biomedical Research
|
| Street address |
30 Bond Street, Office Location: 209 Victoria Street, 5th floor, 516
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5B 1W8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL21278 |
| Series (1) |
| GSE117461 |
Analysis of gene expression in wildtype and PPARdelta knockout naïve CD4+ T cells |
|
