|
Status |
Public on Jul 21, 2018 |
Title |
knockout mouse #3 - non-stimulated |
Sample type |
RNA |
|
|
Source name |
CD4+ T cells
|
Organism |
Mus musculus |
Characteristics |
activation conditions: frozen upon isolation strain: mixed B6/129 age: Young adult cell type: Naïve (CD62Lhi) CD4+ T cell
|
Growth protocol |
T cells were cultured at 0.2 million cells per well in 96 well flat bottomed plates in complete media. Plates were pre-coated with anti-CD3 and anti-CD28
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Qiagen RNA easy kits
|
Label |
Cy3
|
Label protocol |
Illumina TotalPrep-96 Amplification kit (Ambion) was used as per manufacturer's protocol.
|
|
|
Hybridization protocol |
Mouse WG-6 v2.0 BeadChips were incubated at 58oC, with rotation speed 5 for 18 hours.
|
Scan protocol |
BeadChips were scanned on the iScan (Illumina).
|
Description |
KO biological replicate #2-non-stim
|
Data processing |
Data files were quanitified in GenomeStudio v 2010.1 (Illumina). Quantile normalization was performed using GeneSpring v12.1 (Agilent).
|
|
|
Submission date |
Jul 20, 2018 |
Last update date |
Jul 21, 2018 |
Contact name |
Shannon Dunn |
E-mail(s) |
Shannon.Dunn@unityhealth.to
|
Organization name |
St. Michael's Hospital
|
Department |
Keenan Research Centre for Biomedical Research
|
Street address |
30 Bond Street, Office Location: 209 Victoria Street, 5th floor, 516
|
City |
Toronto |
State/province |
ON |
ZIP/Postal code |
M5B 1W8 |
Country |
Canada |
|
|
Platform ID |
GPL21278 |
Series (1) |
GSE117461 |
Analysis of gene expression in wildtype and PPARdelta knockout naïve CD4+ T cells |
|