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Sample GSM3262712 Query DataSets for GSM3262712
Status Public on Sep 23, 2018
Title Tpath2_RNA
Sample type SRA
Source name Five-day cultured Tpath2 cells generated from primary splenic ST2hi memory Th2 cells
Organism Mus musculus
Characteristics cell type: Tpath2
strain: BALB/c
tissue: Tpath2 cells
Extracted molecule total RNA
Extraction protocol Total cellular RNA was extracted with TRIzol reagent (Invitrogen).
TruSeq Stranded mRNA Library Preparation Kit (Illumina) was used according to the manufacturer's protocol.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Description Splenic memory Th2 cells were culture under conditions (10 U/ml IL-7, 10 ng/ml IL-25, 10ng/ml IL-33) for 5 days in vitro.
Data processing Read sequences (50 bp) were aligned to the mm10 mouse reference genome (University of California, Santa Cruz (UCSC) December 2011) using Bowtie (version 0.12.8) and TopHat (version 1.3.2).
Fragments per kilobase of exon per million mapped reads (FPKM) for each gene were calculated using Cufflinks (version 2.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: Text files include FPKM values for each Sample
Submission date Jul 10, 2018
Last update date Sep 24, 2018
Contact name Yusuke ENDO
Phone 81432262186
Organization name Chiba University
Department Immunology
Lab Immunology
Street address 1-8-1 Inohana, Chuo-ku
City Chiba-city
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
Platform ID GPL18480
Series (1)
GSE116842 DUSP10 constrains innate IL-33-mediated cytokine production in ST2hi memory-type pathogenic Th2 cells
BioSample SAMN09635572
SRA SRX4372641

Supplementary file Size Download File type/resource
GSM3262712_Tpath2_RNA.txt.gz 131.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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