|
| Status |
Public on Sep 23, 2018 |
| Title |
Tpath2_RNA |
| Sample type |
SRA |
| |
|
| Source name |
Five-day cultured Tpath2 cells generated from primary splenic ST2hi memory Th2 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Tpath2
strain: BALB/c
tissue: Tpath2 cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was extracted with TRIzol reagent (Invitrogen).
TruSeq Stranded mRNA Library Preparation Kit (Illumina) was used according to the manufacturer's protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
Splenic memory Th2 cells were culture under conditions (10 U/ml IL-7, 10 ng/ml IL-25, 10ng/ml IL-33) for 5 days in vitro.
|
| Data processing |
Read sequences (50 bp) were aligned to the mm10 mouse reference genome (University of California, Santa Cruz (UCSC) December 2011) using Bowtie (version 0.12.8) and TopHat (version 1.3.2).
Fragments per kilobase of exon per million mapped reads (FPKM) for each gene were calculated using Cufflinks (version 2.0.2).
Genome_build: mm10
Supplementary_files_format_and_content: Text files include FPKM values for each Sample
|
| |
|
| Submission date |
Jul 10, 2018 |
| Last update date |
Sep 24, 2018 |
| Contact name |
Yusuke ENDO |
| E-mail(s) |
san3tamariayuyu@chiba-u.jp
|
| Phone |
81432262186
|
| Organization name |
Chiba University
|
| Department |
Immunology
|
| Lab |
Immunology
|
| Street address |
1-8-1 Inohana, Chuo-ku
|
| City |
Chiba-city |
| State/province |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE116842 |
DUSP10 constrains innate IL-33-mediated cytokine production in ST2hi memory-type pathogenic Th2 cells |
|
| Relations |
| BioSample |
SAMN09635572 |
| SRA |
SRX4372641 |
