|
| Status |
Public on Oct 01, 2008 |
| Title |
SFO Water deprivation 2 |
| Sample type |
RNA |
| |
|
| Source name |
SFO Water deprivation
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: Sprague Dawley
Gender: Male
Age: 10-12 weeks
Tissue: Subfornical Organ
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue was dissected in an RNase free manner and stored in RNAlater (Ambion) at -20C for no more than 1 month. Homogenisation in QIAzol was conducted mechanically and the aqueous phase separated using a PLG (Eppendorf). RNA was further purified using RNeasy Minelute cleanup kit (Qiagen). RNA quality was confirmed using nanodrop and bioanylizer 2100 (Agilent)
|
| Label |
Amino allyl UTP (biotin)
|
| Label protocol |
MessegeAmp II aRNA amplification kit (Ambion). Protocol available at http://ambion.com/catalog/CatNum.php?1753
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
Sample represents the Subfornical organ from 5 water deprived SD animals
|
| Data processing |
GCOS version 1.2
|
| |
|
| Submission date |
Sep 30, 2008 |
| Last update date |
Sep 30, 2008 |
| Contact name |
DAVID MURPHY |
| Organization name |
University of Bristol
|
| Department |
Medicine and Dentistry
|
| Lab |
Laboratories for Integrative Neuroscience and Endocrinolgy
|
| Street address |
Whitson Street
|
| City |
Bristol |
| State/province |
AVON |
| ZIP/Postal code |
BS1 3NY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE12978 |
Microarray Analysis of the transcriptome of the Subfornical Organ in the rat |
|
