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Sample GSM3242863 Query DataSets for GSM3242863
Status Public on Mar 31, 2019
Title Human AM from ARDS day 4 [AM_B2_9]
Sample type RNA
 
Source name Human alveolar macrophage
Organism Homo sapiens
Characteristics disease state: Acute respiratory distress syndrome
patient identification number: ARDS patient number 9
age: 19
gender: Male
cell type: alveolar macrophages
time point: Day 4
ventilator-free days (vfd): 0
Treatment protocol None
Growth protocol Human bronchoalveolar lavage fluid (BALF) was collected from ARDS patients and AMs were isolated by antibody-based negative selection via magnetic autoMacs columns (Miltenyi).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by ABI Prism 6100 Nucleic Acid PrepStation (Applied Biosciences, Foster City, CA).
Label Biotin
Label protocol High quality RNA samples were converted to cDNA and biotin-labeled. MessageAmp II-96 Kit, Version C amplification Kit (Applied Biosciences, Foster City, CA).
 
Hybridization protocol Samples were hybridized to the Illumina HumanRef-8 BeadChip v3.
Scan protocol Illumina iScan system.
Description AM_B2_9
Data processing Microarray data was assessed for quality, followed by variance stabilization and quantile normalization of log-transformed values using the Bioconductor package "lumi." The normalized intensities were converted into natural scale.
 
Submission date Jul 02, 2018
Last update date Oct 19, 2022
Contact name Sina Gharib
E-mail(s) sagharib@uw.edu
Organization name University of Washington
Department Medicine
Street address 850 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL6883
Series (1)
GSE116560 Alveolar Macrophage Transcriptional Programs are Associated with Ventilator-Free Days in Acute Respiratory Distress Syndrome

Data table header descriptions
ID_REF
VALUE Natural scale quantile normalized intensities
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 342.05 0.98
ILMN_2055271 434.03 0.06
ILMN_2383229 378.08 0.53
ILMN_1806310 433.81 0.06
ILMN_1779670 446.25 0.04
ILMN_1717783 376.82 0.55
ILMN_1814316 376.67 0.55
ILMN_2359168 379.60 0.51
ILMN_1731507 364.15 0.82
ILMN_1787689 402.35 0.20
ILMN_1745607 396.86 0.25
ILMN_2136495 359.96 0.88
ILMN_1668111 361.69 0.86
ILMN_2295559 387.13 0.39
ILMN_1735045 386.58 0.39
ILMN_1680754 403.65 0.19
ILMN_2375184 376.75 0.55
ILMN_1659452 370.86 0.67
ILMN_1767388 373.75 0.61
ILMN_1675204 378.82 0.52

Total number of rows: 24526

Table truncated, full table size 604 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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