|
| Status |
Public on Mar 31, 2019 |
| Title |
Human AM from ARDS day 4 [AM_B2_40] |
| Sample type |
RNA |
| |
|
| Source name |
Human alveolar macrophage
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Acute respiratory distress syndrome
patient identification number: ARDS patient number 40
age: 50
gender: Male
cell type: alveolar macrophages
time point: Day 4
ventilator-free days (vfd): 0
|
| Treatment protocol |
None
|
| Growth protocol |
Human bronchoalveolar lavage fluid (BALF) was collected from ARDS patients and AMs were isolated by antibody-based negative selection via magnetic autoMacs columns (Miltenyi).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by ABI Prism 6100 Nucleic Acid PrepStation (Applied Biosciences, Foster City, CA).
|
| Label |
Biotin
|
| Label protocol |
High quality RNA samples were converted to cDNA and biotin-labeled. MessageAmp II-96 Kit, Version C amplification Kit (Applied Biosciences, Foster City, CA).
|
| |
|
| Hybridization protocol |
Samples were hybridized to the Illumina HumanRef-8 BeadChip v3.
|
| Scan protocol |
Illumina iScan system.
|
| Description |
AM_B2_40
|
| Data processing |
Microarray data was assessed for quality, followed by variance stabilization and quantile normalization of log-transformed values using the Bioconductor package "lumi." The normalized intensities were converted into natural scale.
|
| |
|
| Submission date |
Jul 02, 2018 |
| Last update date |
Oct 19, 2022 |
| Contact name |
Sina Gharib |
| E-mail(s) |
sagharib@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Medicine
|
| Street address |
850 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE116560 |
Alveolar Macrophage Transcriptional Programs are Associated with Ventilator-Free Days in Acute Respiratory Distress Syndrome |
|
