|
Status |
Public on Mar 31, 2019 |
Title |
Human AM from ARDS day 1 [AM_B1_46] |
Sample type |
RNA |
|
|
Source name |
Human alveolar macrophage
|
Organism |
Homo sapiens |
Characteristics |
disease state: Acute respiratory distress syndrome patient identification number: ARDS patient number 46 age: 18 gender: Female cell type: alveolar macrophages time point: Day 1 ventilator-free days (vfd): 19
|
Treatment protocol |
None
|
Growth protocol |
Human bronchoalveolar lavage fluid (BALF) was collected from ARDS patients and AMs were isolated by antibody-based negative selection via magnetic autoMacs columns (Miltenyi).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated by ABI Prism 6100 Nucleic Acid PrepStation (Applied Biosciences, Foster City, CA).
|
Label |
Biotin
|
Label protocol |
High quality RNA samples were converted to cDNA and biotin-labeled. MessageAmp II-96 Kit, Version C amplification Kit (Applied Biosciences, Foster City, CA).
|
|
|
Hybridization protocol |
Samples were hybridized to the Illumina HumanRef-8 BeadChip v3.
|
Scan protocol |
Illumina iScan system.
|
Description |
AM_B1_46
|
Data processing |
Microarray data was assessed for quality, followed by variance stabilization and quantile normalization of log-transformed values using the Bioconductor package "lumi." The normalized intensities were converted into natural scale.
|
|
|
Submission date |
Jul 02, 2018 |
Last update date |
Oct 19, 2022 |
Contact name |
Sina Gharib |
E-mail(s) |
sagharib@uw.edu
|
Organization name |
University of Washington
|
Department |
Medicine
|
Street address |
850 Republican St.
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109 |
Country |
USA |
|
|
Platform ID |
GPL6883 |
Series (1) |
GSE116560 |
Alveolar Macrophage Transcriptional Programs are Associated with Ventilator-Free Days in Acute Respiratory Distress Syndrome |
|