|
| Status |
Public on Nov 14, 2008 |
| Title |
PBMCs_Control_paired_rep4 (HG-U133B) |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs, Control, Sample 2115
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: male, Race: Caucasian
|
| Treatment protocol |
as described in Wendy T. Watford, et al., Tpl2 kinase regulates T cell interferon gamma production and host resistance to Toxoplasma gondii. Journal of Experimental Medicine.
|
| Growth protocol |
as described in Wendy T. Watford, et al., Tpl2 kinase regulates T cell interferon gamma production and host resistance to Toxoplasma gondii. Journal of Experimental Medicine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cytokine-stimulated cells were lysed in RNeasy lysis buffer (Qiagen, Valencia, CA) and total RNA was isolated with the Qiagen RNeasy Midi kit using the standard protocol. RNA was purified using Trizol (Invitrogen Life Technologies, Carlsbad, CA) and chloroform, followed by ethanol precipitation.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was synthesized using the BioArray High Yield RNA transcript labeling kit (Enzo Life Sciences, Inc., Farmingdale, NY) Affymetrix).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix Human Genome-U133A and HG-U133B microarray chips according to the manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station Model 400.
|
| Scan protocol |
GeneChips were scanned by the HP-Affymetrix GeneArray Scanner using the manufacturer's protocol.
|
| Description |
PBMC's were isolated from 7 healthy NIH blood bank donors.
|
| Data processing |
The data were analyzed with Microarray Suite version 5 (MAS5) using Affymetrix default analysis settings and global scaling as the normalization method. The trimmed mean (2% trim at bottom and at top) target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Sep 18, 2008 |
| Last update date |
Nov 14, 2008 |
| Contact name |
Alan E. Berger |
| E-mail(s) |
alanberger@alum.mit.edu
|
| Phone |
301-938-3565
|
| Organization name |
Johns Hopkins University
|
| Department |
School of Medicine
|
| Lab |
Division of Allergy and Clinical Immunology
|
| Street address |
5501 Hopkins Bayview Circle Room 3B.74C
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (1) |
| GSE12839 |
Expression data from human PBMC samples exposed to IL-12 and paired controls |
|
