|
Status |
Public on Sep 03, 2008 |
Title |
Xenopus borealis Testis, biological rep 3 |
Sample type |
RNA |
|
|
Source name |
Xenopus borealis, sexually mature testis
|
Organism |
Xenopus borealis |
Characteristics |
Adult male Xenopus borealis (Kenya)
|
Treatment protocol |
Animal was immersed in MS222 and gonad was immediately extracted
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using TRIzol® Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol and purified with RNeasy Mini Kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Two micrograms of total RNA was used to prepare biotin labeled cRNA probes following the manufacturer’s (Affymetrix) protocol.
|
|
|
Hybridization protocol |
Hybridization to Affymetrix Xenopus laevis Genome expression array followed the manufacturer's (Affymetrix) protocol.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
1 of 3 biological replicates of gene expression data from sexually mature testis.
|
Data processing |
Data were RMA normalized using RMA Express and a probemask (based on perfect match probes for X.laevis and X.borealis).
Normalization of 3 biological replicates were performed with 3 biological replicates of X.borealis brain
|
|
|
Submission date |
Aug 29, 2008 |
Last update date |
Sep 02, 2008 |
Contact name |
Frederic Chain |
E-mail(s) |
chainfj@mcmaster.ca
|
Organization name |
McMaster University
|
Department |
Biology
|
Lab |
LSB 324
|
Street address |
1280 Main Street West
|
City |
Hamilton |
State/province |
Ontario |
ZIP/Postal code |
L8S4K1 |
Country |
Canada |
|
|
Platform ID |
GPL1318 |
Series (1) |
GSE12625 |
Expression data for a study on cross-species hybridization on single-species microarrays |
|