GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM316732 Query DataSets for GSM316732
Status Public on Sep 03, 2008
Title Xenopus borealis Testis, biological rep 1
Sample type RNA
Source name Xenopus borealis, sexually mature testis
Organism Xenopus borealis
Characteristics Adult male Xenopus borealis (Kenya)
Treatment protocol Animal was immersed in MS222 and gonad was immediately extracted
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol® Reagent (Invitrogen Life Technologies) according to the manufacturer’s protocol and purified with RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Two micrograms of total RNA was used to prepare biotin labeled cRNA probes following the manufacturer’s (Affymetrix) protocol.
Hybridization protocol Hybridization to Affymetrix Xenopus laevis Genome expression array followed the manufacturer's (Affymetrix) protocol.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description 1 of 3 biological replicates of gene expression data from sexually mature testis.
Data processing Data were RMA normalized using RMA Express and a probemask (based on perfect match probes for X.laevis and X.borealis).
Normalization of 3 biological replicates were performed with 3 biological replicates of X.borealis brain
Submission date Aug 29, 2008
Last update date Sep 02, 2008
Contact name Frederic Chain
Organization name McMaster University
Department Biology
Lab LSB 324
Street address 1280 Main Street West
City Hamilton
State/province Ontario
ZIP/Postal code L8S4K1
Country Canada
Platform ID GPL1318
Series (1)
GSE12625 Expression data for a study on cross-species hybridization on single-species microarrays

Data table header descriptions
VALUE RMA normalized (log2) using RMA express using "XB+XL perfect match probemask"

Data table
Xl.1.1.S1_s_at 12.75483702
Xl.10012.1.A1_at 7.35488238
Xl.10032.1.A1_s_at 9.708455509
Xl.10043.1.A1_at 6.390848579
Xl.10043.2.S1_at 3.733300862
Xl.10054.1.S1_at 9.138249633
Xl.10087.1.A1_at 8.869773201
Xl.10104.1.A1_at 5.861101649
Xl.10118.1.S1_at 5.580789276
Xl.1013.1.S1_at 6.947878338
Xl.10142.1.S1_at 7.653754204
Xl.1015.1.S1_at 8.944284207
Xl.10170.1.A1_at 7.868770018
Xl.10191.1.S1_at 10.00866732
Xl.10208.1.S1_at 6.824108041
Xl.10222.1.A1_at 5.689336206
Xl.10227.1.A1_at 7.955697299
Xl.10230.1.A1_at 8.131684588
Xl.10251.1.A1_at 5.415330134
Xl.10287.1.S1_at 8.557239623

Total number of rows: 2143

Table truncated, full table size 59 Kbytes.

Supplementary file Size Download File type/resource
GSM316732.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap