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Sample GSM3136759 Query DataSets for GSM3136759
Status Public on Jun 20, 2018
Title MBC12
Sample type RNA
Source name Breast
Organism Homo sapiens
Characteristics disease state: medullary breast carcinoma
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
Data processing Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
Submission date May 08, 2018
Last update date Jun 20, 2018
Contact name Cecile Laurent
Organization name Institut Curie
Street address 26 rue d'ulm
City Paris Cedex 05
ZIP/Postal code 75248
Country France
Platform ID GPL570
Series (2)
GSE114168 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression.
GSE114269 Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness

Data table header descriptions
VALUE RMA signal intensity.

Data table
1007_s_at 10.0935638909947
1053_at 7.26908591099395
117_at 6.88173533275524
121_at 7.87780686888668
1255_g_at 3.26190907343464
1294_at 7.78089014286172
1316_at 4.85442481299168
1320_at 4.33212371932492
1405_i_at 9.34044628160773
1431_at 3.73925906079964
1438_at 9.55836238267954
1487_at 7.70564538071861
1494_f_at 5.35238223887259
1552256_a_at 9.97826843281407
1552257_a_at 8.94144480942254
1552258_at 4.70028159511166
1552261_at 5.44526416329467
1552263_at 5.991262329988
1552264_a_at 7.94316859829373
1552266_at 4.89981299479851

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.

Supplementary file Size Download File type/resource
GSM3136759_TM01_ARN0021_s1h1s1_U133p2.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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