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Sample GSM3110585 Query DataSets for GSM3110585
Status Public on Apr 26, 2018
Title HBL-1 treated by APRIL replicate 1
Sample type RNA
 
Source name HBL-1 treated by APRIL
Organism Homo sapiens
Characteristics cell line: HBL-1
cell type: B Lymphoma cell line
treated with: recombinant purified APRIL (1µg/ml) for 6hrs
Treatment protocol BJAB and HBL-1 cell lines were stimulated for 6 hours with recombinant purified APRIL (1µg/ml) or with EDA (1µg/ml) as control.
Growth protocol BJAB and HBL-1 cell lines were cultured in RPMI1640 medium and 10% FCS.
Extracted molecule total RNA
Extraction protocol Total RNAs were purified from cells with the RNeasy Qiagen purification kit following manufacturer's instructions including an on-column DNase I treatment step to remove any residual contaminant DNA.
Label biotin
Label protocol Standard Affymetrix procedures
 
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description BH3
RNA expression data from HBL-1 treated by APRIL
Data processing Data obtained for the whole set of samples were normalized by RMA.
affymetrix-algorithm-name = sst-rma-gene-full
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.4.1.46
 
Submission date Apr 25, 2018
Last update date Apr 26, 2018
Contact name Jerome Moreaux
E-mail(s) jerome.moreaux@igh.cnrs.fr
Organization name CHRU Montpellier
Department Biological Hematology
Lab Laboratory for Monitoring Innovative Therapies
Street address 80 Av. Augustin Fliche
City Montpellier
ZIP/Postal code 34295
Country France
 
Platform ID GPL23126
Series (1)
GSE113643 Coreceptor delivery of paracrine APRIL to Golgic BCMA lowers immunogenicity of B-cell lymphoma

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
AFFX-BkGr-GC03_st 10.3957
AFFX-BkGr-GC04_st 7.99719
AFFX-BkGr-GC05_st 7.28872
AFFX-BkGr-GC06_st 6.51989
AFFX-BkGr-GC07_st 5.81655
AFFX-BkGr-GC08_st 5.22545
AFFX-BkGr-GC09_st 4.79766
AFFX-BkGr-GC10_st 4.34482
AFFX-BkGr-GC11_st 3.93395
AFFX-BkGr-GC12_st 3.53776
AFFX-BkGr-GC13_st 3.06598
AFFX-BkGr-GC14_st 2.84787
AFFX-BkGr-GC15_st 2.72157
AFFX-BkGr-GC16_st 2.7232
AFFX-BkGr-GC17_st 2.6838
AFFX-BkGr-GC18_st 2.71462
AFFX-BkGr-GC19_st 2.89916
AFFX-BkGr-GC20_st 2.93561
AFFX-BkGr-GC21_st 3.09294
AFFX-BkGr-GC22_st 3.59997

Total number of rows: 138745

Table truncated, full table size 3533 Kbytes.




Supplementary file Size Download File type/resource
GSM3110585_BH3.CEL.gz 25.5 Mb (ftp)(http) CEL
GSM3110585_BH3_Clariom_D_Human_.sst-rma-gene-full.chp.gz 888.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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